/ AbstractThe PLanetary Transits and Oscillations of stars (PLATO) mission will begin its four-year nominal mission in early 2027 by monitoring its first Long-duration Observation Phase field at South (LOPS2), for at least two years continuously. The primary aim of PLATO is a very ambitious and challenging one: the discovery of Earth-like planets in the habitable zone of nearby and bright solar analogues. To this purpose, the PLATO Mission Consortium, through its Ground-based Observing Program (GOP), will perform the follow-up needed to confirm part of the candidate planets photometrically detected by PLATO and measure their masses through radial velocity curves. For the LOPS2, the GOP is committed (as part of the PLATO mission) to follow-up the candidate exoplanets discovered orbiting the 15,000 high-quality target subset of the PLATO Input Catalog (PIC) known as the Prime Sample (PS). The PS will be made public nine months before launch in the context of the first Guest Observer (GO) call for proposals to be issued by the European Space Agency (ESA). Here, we present the quantitative metrics and thresholds defined to select and prioritize the PS. Our method is perfectly general and suitable to rank any list of stars surveyed for transiting planets. We also describe the astrophysical properties of the LOPS2 PS, both in a statistical sense and for some specific targets of interest.