/ AbstractWe report a measurement of the antineutrino rate from the fission of U-235 with the STEREO detector using 119 days of reactor turned on. In our analysis, we perform several detailed corrections and achieve the most precise single measurement at reactors with highly enriched U-235 fuel. We measure an IBD cross section per fission of $σ_f$ = (6.34 $\pm$ 0.06 [stat] $\pm$ 0.15 [sys] $\pm$ 0.15 [model]) $\times$ 10${}^{-43}$ cm${}^{2}$/fission and observe a rate deficit of (5.2 $\pm$ 0.8 [stat] $\pm$ 2.3 [sys] $\pm$ 2.3 [model])% compared to the model, consistent with the deficit of the world average. Testing U-235 as the sole source of the deficit, we find a tension between the results of lowly and highly enriched U-235 fuel of 2.1 standard deviations.