Science requirements for EAGLE for the E-ELT

We present an overview of the EAGLE science case, which spans spatially resolved spectroscopy of targets from five key science areas - ranging from studies of heavily obscured Galactic star clusters, right out to the first galaxies at the highest redshifts. Here we summarise the requirements adopted for the study and also evaluate the availability of natural guide stars in example fields, which will impact on the adaptive optics performance and architecture.